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KMID : 0358920140410040292
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Journal of the Korean Academy of Pedodontics
2014 Volume.41 No. 4 p.292 ~ p.297
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Rapid Detection of Pathogens Associated with Dental Caries and Periodontitis by PCR Using a Modified DNA Extraction Method
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Kim Jae-Hwan
Kim Mi-Ah
Lee Daw-Woo
Baik Byeong-Ju
Yang Yeon-Mi
Kim Jae-Gon
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Abstract
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DNA extraction is a prerequisite for the identification of pathogens in clinical samples. Commercial DNA extraction kits generally involve time-consuming and laborious multi-step procedures. In the present study, our modified DNA isolation method for saliva samples allows for the quick detection of pathogens associated with dental caries or periodontitis by PCR within 1 h. To release DNA from the bacteria, 1 min of boiling was adequate, and the resulting isolated DNA can be used many times and is suitable for long term storage of at least 13 months at 4℃, and even longer at -20℃. In conclusion, our modified DNA extraction method is simple, rapid, and cost-effective, and suitable for preparing DNA from clinical samples for PCR for the rapid detection of oral pathogens from saliva.
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KEYWORD
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DNA extraction, Oral pathogens, Saliva, Polymerase chain reaction(PCR)
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